Composite

Part:BBa_K1403018:Design

Designed by: Cristina Garcia-Timermans   Group: iGEM14_Paris_Bettencourt   (2014-10-06)


Expression of agaA in E coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 372
    Illegal BamHI site found at 603
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

agaA sequence codon optimized for E coli 12 (IDT online tool). We checked with Generious that there were no restriction sites for EcoRI, SpeI, XbaI and PstI.



Source

It is the agaA sequence codon optimized for E coli.

References